Home > IV Asian Horticultural Congress – AHC2023 > Expression analysis of anthocyanin biosynthesis-related genes in bicolor Echinacea ‘Pink Eye’

Articles

Expression analysis of anthocyanin biosynthesis-related genes in bicolor Echinacea ‘Pink Eye’

Article number

1404_116

Pages

855 – 860

Language

English

Abstract

Echinacea purpurea has reddish-purple flowers (ray-florets), which are colored by cyanidin-derived anthocyanins.
Some Echinacea cultivars have bicolor flowers, however, its molecular mechanism has been unknown.
Therefore, we investigated the molecular mechanism of the difference in flower coloration using the bicolor cultivar ‘Pink Eye’, which has deep-pink and white ray-florets.
UPLC/MS/MS analysis showed that two anthocyanins are the main component of flower coloration in deep-pink regions, as same as wild-type.
In the white regions, the amount of anthocyanins decreased and the amount of flavonols increased comparing with the pink regions.
From these results, we concluded that anthocyanin biosynthesis-related gene(s) would be mutated in white regions.
To determine the mutated factors, we compared the expression of flavonoid 3’-hydroxylase (F3’H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) genes between deep-pink regions and white regions.
The expression of F3’H was not different between two regions, while the expressions of DFR and ANS were severely decreased in white regions.
Sequence analysis showed that both gene structures were not different in each region.
These results suggested that epigenetic mutation or mutation of transcription regulator(s) would be occurred in white regions of ‘Pink Eye’.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

M. Kojima, N. Noda, T. Sakagami, Y. Akita

Keywords

cone flower, flavonoid, flower coloration, transcription

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.116

Groups involved

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183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

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184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani