Articles

Development of a database program for managing genotype information and simulating segregation patterns of horticulturally important genes in apple

Article number
1404_85
Pages
625 – 630
Language
English
Abstract
Over the last two decades, numerous DNA markers useful in breeding programs in domesticated apple (Malus × domestica Borkh), have been developed through genomic research.
Marker-assisted breeding (MAB) or genomics-assisted breeding (GAB) has been employed in breeding programs on the ground.
They involve screening of the available genotypes for specific DNA markers, which can then be applied for the selection of parents while making crosses.
However, this selection is often managed by breeders on spreadsheets, as there is no small-scale program available for storing that information with a user-friendly interface.
Moreover, for efficient use of farm resources, it is important to estimate the exact number of seedlings to be raised.
To address these issues, we developed a database program named Electric Record Assistant for Breeding-Genotype (ERAB-Genotype). The database can store genotypic information for gene/DNA markers often used in scion apple breeding, such as disease resistance, fruit quality, cross compatibility, and recessive lethality.
The simulation function of the program generates probable segregation patterns of each gene/trait and estimates the proportion of individuals that can fulfill the selection criteria.
Inbreeding coefficient values of available cultivars and possible cross combinations are also displayed.
ERAB-Genotype helps breeders in designing cross combinations for an efficient marker-assisted selection strategy and avoidance of inbreeding depression.

Publication
Authors
S. Moriya, T. Shimizu, A. Hori, Y. Sawamura
Keywords
apple, cross combination, inbreeding coefficient, marker-assisted selection, simulation
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