Home > IV Asian Horticultural Congress – AHC2023 > Embryo age and culture medium determine the efficacy of embryo rescue in hybridizations between Crossostephium chinense (L.) Makino and Chrysanthemum spp.

Articles

Embryo age and culture medium determine the efficacy of embryo rescue in hybridizations between Crossostephium chinense (L.) Makino and Chrysanthemum spp.

Article number

1404_94

Pages

683 – 692

Language

English

Abstract

Chrysanthemum is one of the most important ornamental flowers cultivated worldwide.
However, soil salinization has seriously hindered the development of chrysanthemum industry. Crossostephium chinense, a salt tolerant species closely related to Chrysanthemum genus, can be used for developing salt tolerant germplasm in cultivated chrysanthemums.
In this study, Crossostephium chinense was introduced to hybridize with three Chrysanthemum plant materials.
Pollen germination and pollen tube elongation were examined to assess the potential hybridization obstacles in these intergeneric hybrids.
Subsequently, putative hybrids were obtained through embryo rescue techniques.
They were further examined through the morphology and cytology investigations.
Our result found that there are no pre-fertilization obstacles in these intergeneric hybridizations.
Through embryo rescue, the suitable time for inoculation was 18 days after pollination, and the most suitable media combinations for differentiation were MS+6-BA 2.0 mg L^‑1+NAA 0.2 mg L^‑1. Finally, the true hybrids were successfully identified by combining morphology and cytology.
The developed procedure has the potential to yield new intergeneric hybrids with strong salt-tolerance inherited from Crossostephium chinense. These comprehensive technologies used in this study may serve as valuable references for overcoming hybridization obstacles among Chrysanthemum related genera.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

H. Li, J. Zhong, Z.Y. Guo, Y.X. Wang, Y.F. Lu, D.W. Li, T.C. Zheng, M. Sun

Keywords

chrysanthemum, salt tolerance, intergeneric hybridization, hybrid identification

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.94

Groups involved

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F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani