Articles
Quantitative trait locus analysis of the F1 breeding population of 180-1-08 × ‘Del Monte Gold’ pineappleses
Article number
1404_17
Pages
123 – 128
Language
English
Abstract
Pineapples (Ananas comosus L.) are widely cultivated in tropical and subtropical regions including in Okinawa, Japan.
In Okinawa, DNA marker assisted selection (MAS) has been employed for selecting qualitative traits in pineapples.
However, quantitative trait locus (QTL) based MAS has not yet been utilized as QTLs in pineapples were previously unidentified.
To proceed with effective MAS for breeding selection, QTLs and their associated effects must be identified.
In this study, we performed restriction-site associated sequencing (RAD-Seq) and QTL analysis for 108 individuals from the F1 population of the pineapple breeding line 180-1-08 × ‘Del Monte Gold’ For QTL analysis, 20 traits were evaluated – fruit flesh color (visual observation, L*, a*, b*), carotenoid contents (violaxanthin, cis-violaxanthin, lutein,
-cryptoxanthin, 
-carotene, zeaxanthin, -carotene, and total carotenoid), harvest day, slip number, stem shoot number, sucker number, fruit weight, soluble solids content, acidity, and ascorbic acid content.
All traits were evaluated in 2016, without replications.
Double digest RAD-Seq analysis and genetic linkage map construction using Lep-map3 software generated a saturated linkage map of 4,082 loci with 63,238 single nucleotide polymorphisms in 26 linkage groups, which covered 25 pseudomolecules in the pineapple genome.
QTL analysis detected color related QTLs; fruit color a* (detected on chromosome 4),-cryptoxanthin content (chromosome 9, 13 and 14), -carotene content (chromosome 4 and 6), violaxantnin (chromosome 6), and lutein (chromosome 2). The QTL information identified in this study could contribute to effective marker-assisted selection for enhanced fruit quality.
In Okinawa, DNA marker assisted selection (MAS) has been employed for selecting qualitative traits in pineapples.
However, quantitative trait locus (QTL) based MAS has not yet been utilized as QTLs in pineapples were previously unidentified.
To proceed with effective MAS for breeding selection, QTLs and their associated effects must be identified.
In this study, we performed restriction-site associated sequencing (RAD-Seq) and QTL analysis for 108 individuals from the F1 population of the pineapple breeding line 180-1-08 × ‘Del Monte Gold’ For QTL analysis, 20 traits were evaluated – fruit flesh color (visual observation, L*, a*, b*), carotenoid contents (violaxanthin, cis-violaxanthin, lutein,
-cryptoxanthin, -carotene, zeaxanthin, -carotene, and total carotenoid), harvest day, slip number, stem shoot number, sucker number, fruit weight, soluble solids content, acidity, and ascorbic acid content.All traits were evaluated in 2016, without replications.
Double digest RAD-Seq analysis and genetic linkage map construction using Lep-map3 software generated a saturated linkage map of 4,082 loci with 63,238 single nucleotide polymorphisms in 26 linkage groups, which covered 25 pseudomolecules in the pineapple genome.
QTL analysis detected color related QTLs; fruit color a* (detected on chromosome 4),
-cryptoxanthin content (chromosome 9, 13 and 14), -carotene content (chromosome 4 and 6), violaxantnin (chromosome 6), and lutein (chromosome 2). The QTL information identified in this study could contribute to effective marker-assisted selection for enhanced fruit quality.
Publication
Authors
K. Nashima, M. Takeuchi, Y. Omine, N. Urasaki, K. Tarora, A. Irei, M. Shoda, Y. Nishiba, T. Sugawara, T. Sato, S. Kiriyama, M. Yamada, M. Kunihisa, C. Nishitani, T. Yamamoto
Keywords
carotenoid, flesh color, NGS, SNPs
Groups involved
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Tropical and Subtropical Fruit and Nuts
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Plant-Environment Interactions in Field Systems
- Division Greenhouse and Indoor Production Horticulture
- Division Postharvest and Quality Assurance
- Division Precision Horticulture and Engineering
- Division Horticulture for Human Health
- Division Sustaining Horticulture in a Changing World
- Division Landscape and Urban Horticulture
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