Home > IV Asian Horticultural Congress – AHC2023 > Evaluation of Bacillus spp. for biological control of Fusarium stem rot in Anoectochilus formosanus

Articles

Evaluation of Bacillus spp. for biological control of Fusarium stem rot in Anoectochilus formosanus

Article number

1404_137

Pages

1001 – 1008

Language

English

Abstract

Anoectochilus formosanus Hayata is an orchid rich in bioactive compounds with significant medicinal properties.
The stem rot disease caused by Fusarium oxysporum is a major challenge that can cause significant yield losses of A. formosanus Hayata.
Only a few fungicides and non-chemical agents are available for controlling this disease.
Therefore, this study aims to explore the biological agents for their potential to control Fusarium stem rot.
Several microbial strains were isolated and screened from rhizosphere soil samples of A. formosanus Hayata.
Dual culture assay indicated four isolates, AFA5, AFB4, AFC4, and AFC5, demonstrating significant antagonistic effects against the F. oxysporum mycelial growth, with an inhibition rate above 55.9%. In addition, these isolates could effectively inhibit F. oxysporum spore germination, with AFA5 exhibiting the highest inhibition rate of 97.7%. Using 16S rDNA and gyrB gene sequencing analysis, these four antagonistic bacteria were identified as Bacillus amyloliquefaciens. Moreover, we discovered that these antagonistic bacteria secrete multiple extracellular enzymes, including protease, amylase, cellulase, and lipase, while enhancing phosphate solubilization.
Further, the suspension of B. amyloliquefaciens isolates was directly applied onto A. formosanus Hayata roots before spore inoculation, resulting in a 35.0-43.3% reduction of Fusarium stem rot disease incidence.
In the pot experiments, the treatment group drenched with bacterial suspension effectively demonstrated a 14.6 to 20.8% reduction in A. formosanus Hayata mortality rate.
In conclusion, this study successfully identified four Bacillus amyloliquefaciens strains from rhizosphere soil, exhibiting a significant potential as bio-control agents for Fusarium stem rot disease in Anoectochilus formosanus Hayata.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

C. Chen, C.Y. Lu, R.M. Hsieh, G.M. Yang

Keywords

antagonism, biocontrol agent, Fusarium oxysporum, Orchidaceae, rhizosphere microbiome

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.137

Groups involved

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P.A. Casquero | A. Porteous | C. Domínguez | A. Rodríguez-González | S. Mayo-Prieto

181. Effects of rain-protected culture in a plastic greenhouse using the whole harvest cultivation method for one-year-old asparagus plants

S. Yamashita | K. Seimiya | K. Yamafuji | T. Taguchi | S. Motoki

182. Estimation of the light extinction coefficients of lateral asparagus ferns using a numerical method

T. Kuroyanagi | H. Yoshikoshi | M. Hikawa-Endo

183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani