Home > IV Asian Horticultural Congress – AHC2023 > Bulked sequence analysis identifies two loci controlling anthocyanin accumulation in a red-purple cultivar of Japanese leaf vegetable komatsuna (Brassica rapa L.)

Articles

Bulked sequence analysis identifies two loci controlling anthocyanin accumulation in a red-purple cultivar of Japanese leaf vegetable komatsuna (Brassica rapa L.)

Article number

1404_92

Pages

669 – 676

Language

English

Abstract

We investigated leaf color and anthocyanin content in cross-progeny of two Japanese Brassica rapa crops, a red-purple cultivar of komatsuna (Japanese mustard spinach) and a green cultivar of nabana (young flowering stems of Brassica eaten as a vegetable). The phenotype of all F₁ progeny was intermediate between the parents.
High-performance liquid chromatography analysis revealed the similarity of the anthocyanin compositions between the parent komatsuna and the F₁ plants, indicating that the shade of purple depends on the total amount of anthocyanins.
One hundred and one F₂ plants segregated into green and purple-tinted phenotypes at a ratio of 43:58, suggesting the presence of at least two loci controlling anthocyanin accumulation in the komatsuna parent.
Bulked sequence analysis of genomic DNA bulked by F₂ color identified two loci controlling anthocyanin accumulation around 23 Mb on chromosome A07 (a07-23) and 45 Mb on A09 (a09-45) within the B. rapa reference genome v. 3.0. Genotyping by DNA markers flanking the loci indicated that a07-23 from the red-purple komatsuna is likely identical to a known locus harboring several MYB genes involved in the anthocyanin biosynthesis pathway in pigmented B. rapa vegetables, and that a09-45 is a minor locus complementarily working under certain genetic backgrounds.
Comparative RNA-seq analysis between red-purple komatsuna and green nabana cultivars confirmed that many anthocyanin genes, including two MYB genes within the a07-23 region, were upregulated in the red-purple cultivar, suggesting that these are responsible for constitutive anthocyanin accumulation in the pigmented B. rapa vegetable.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

S. Mizuno, A. Otsuka, Y. Kamiyoshihara, K. Nashima, F. Shinmachi, K. Watanabe, A. Tateishi

Keywords

DNA-seq, genotyping, leaf vegetable, MYB transcription factor, RNA-seq

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.92

Groups involved

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182. Estimation of the light extinction coefficients of lateral asparagus ferns using a numerical method

T. Kuroyanagi | H. Yoshikoshi | M. Hikawa-Endo

183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani