Home > IV Asian Horticultural Congress – AHC2023 > A GRAS-Di system linkage map in Chinese cabbage

Articles

A GRAS-Di system linkage map in Chinese cabbage

Article number

1404_44

Pages

323 – 330

Language

English

Abstract

In Chinese cabbage (Brassica rapa L. var. pekinensis), the hybrid progeny of the selected parental inbred lines exhibits enhanced performance relative to its parents.
This phenomenon is known as hybrid vigor or heterosis.
F₁ hybrid cultivars that increased yield by heterosis are now being widely applied to various horticultural crops.
However, the molecular mechanisms underlying heterosis remain unclear.
To apply quantitative trait locus (QTL) analysis for the heterotic characters using the F₂ population, genotyping by random amplicon sequencing-direct (GRAS-Di) was performed in parental inbred lines of an F₁ hybrid cultivar of Chinese cabbage, ‘W77’, and its F₂ population.
GRAS-Di is a recently developed genotyping technique that is a derivative of amplicon sequencing through the amplification of random primers.
It can identify a large number of genetic markers covering all chromosomes.
GRAS-Di generated 1,998 markers between parental inbred lines of ‘W77’, and genotyping results by GRAS-Di were consistent with those by DNA markers previously developed.
We tested GRAS-Di on the F₂ population of ‘W77’ and developed a linkage map with an 845 cM cumulative genome length including 588 markers.
GRAS-Di technology is a useful tool for genetic studies of Chinese cabbage.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

H. Mehraj, M.A. Akter, T. Takasaki-Yasuda, E.S. Dennis, R. Fujimoto

Keywords

Brassica rapa, DNA marker, genetic study, heterosis, hybrid vigor

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.44

Groups involved

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185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani