Home > IV Asian Horticultural Congress – AHC2023 > Agrobacterium-mediated transformation of rose meristem in vitro

Articles

Agrobacterium-mediated transformation of rose meristem in vitro

Article number

1404_46

Pages

339 – 346

Language

English

Abstract

Rose is one of the most valuable ornamental crops in the world due to its use as cut flowers, potted plants, landscape shrubs, perfume, and food/beverage additives.
Conventional breeding has achieved notable progress in improving important traits of roses, such as plant form, disease resistance, and remontancy.
However, conventional breeding faces challenges due to the complex nature of rose genetics, including factors like polyploidy, high heterozygosity, and low fertility in existing breeding lines.
To overcome these challenges and enable precise trait engineering, the genome editing technique offers a promising solution.
However, efficient regeneration and transformation systems are not available for genome editing in roses.
In this study, we developed an efficient method for Agrobacterium-mediated genetic transformation of in vitro meristem cultures of roses.
Meristems were isolated from in vitro cultured multi-shoots and transformed using A. tumefaciens strain GV3101 carrying a binary vector.
The vector contained a bialaphos resistance (bar) gene and a green fluorescent protein (GFP) reporter gene.
By employing GFP visual selection, we obtained an average of eight putative transgenic cell lines from three independent transformations of 30 meristems.
Transgenic plants were then regenerated through somatic embryogenesis from transgenic callus.
Subsequent PCR analysis demonstrated that 100% of the regenerated plants were transgenic, confirming the success of the transformation process.
The development of this highly efficient transformation method provides a valuable tool for rose transformation and facilitates trait engineering and functional genomics studies in roses.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

K. Da, D. Harmon, A. Nelson, H. Leng, S. McLennan, C. Nix, E. Kinsch, G. Leoshko, G. Hall, D. Huang, D. Touchel, W. Liu, T. Ranney

Keywords

Agrobacterium-mediated transformation, GFP, meristem, Rosa, somatic embryogenesis

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.46

Groups involved

Online Articles (205)

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153. Effects of temperatures on seed germination and initial growth of seedlings in Wisteria floribunda

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154. Effect of humic substances and mycorrhizal symbiosis on growth and heat stress tolerance in everbearing strawberry

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155. Effect of bulbs size and gibberellic acid (GA₃) on growth and flowering of Eucomis

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156. Characteristics of Iris ensata var. spontanea growing along the Tanesashi Coast, Japan: focusing on salt tolerance

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157. Vernalization characteristics in Lavandula × intermedia

158. Large amounts of calcium oxalate crystals accumulate in epidermis of edible cactus (Nopalea cochenillifera)

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159. Promotion of rooting and growth with the changes in functional metabolites in mycorrhizal tea plants

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161. A preliminary study on image-based grading of apples harvested in the southern region of Nagano Prefecture, Japan

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162. Effect of ‘joint cutting’ and positional difference on new shoot growth and uniformity of fruit quality in tree joint training system of Japanese pear ‘Housui’

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163. Productivity and productive age of tree joint training system of Japanese pear as high-density planting cultivation without dwarfing rootstock

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164. Toward an establishment of a deep learning-based flower bud phenotyping system in Prunus mume

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165. The timing of trehalose injection affects the number of flowers and the expression of VvCKX5 in the hybrid grape Vitis labrusca × V. vinifera ‘Shine Muscat’

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169. Effects of environmental conditions for inducing flower bud initiation on plant growth and flowering of June-bearing strawberry grown under LED lighting

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171. Estimation of strawberry fruit weight based on fruit diameter

172. Effect of carbon dioxide application on tomato growth dynamics and yield within a low solar radiation environment in Akita (cold region), Japan

173. Prediction of yield based on fruit set and dry matter production in greenhouse tomato

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174. Effects of environmental control using groundwater and full open-roof vinyl house on yield in long-term cultivation of three cultivars of tomatoes in Akita, one of the coldest regions in Japan

175. Effects of sorghum green manure crop on yield and root zone of ‘Mizunasu’ eggplant

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176. Modeling fruit set in greenhouse sweet pepper

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177. Effect of different leaf area management on growth and yield of cucumber (Cucumis sativus L.) cultivated in hydroponic system

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178. Effect of substrates elaborated with waste of tires and cables in lettuce growth

179. LCA analysis of leaf lettuce production in plant factories in various decarbonization scenarios

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180. Bioaccumulation of heavy metals from substrates elaborated with waste of tires and cables in lettuce

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181. Effects of rain-protected culture in a plastic greenhouse using the whole harvest cultivation method for one-year-old asparagus plants

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182. Estimation of the light extinction coefficients of lateral asparagus ferns using a numerical method

T. Kuroyanagi | H. Yoshikoshi | M. Hikawa-Endo

183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani