Home > IV Asian Horticultural Congress – AHC2023 > Identification and expression analyses of the CENTRORADIALIS (CEN) gene family involved in flowering regulation in persimmon (Diospyros kaki)

Articles

Identification and expression analyses of the CENTRORADIALIS (CEN) gene family involved in flowering regulation in persimmon (Diospyros kaki)

Article number

1404_87

Pages

639 – 644

Language

English

Abstract

Persimmon (Diospyros kaki Thunb.) has been grown for centuries as one of the most important fruit trees in eastern Asia.
As with other fruit tree species, persimmon has a relatively long juvenile period of 3 to 4 years, which hinders effective breeding.
Furthermore, the long juvenile period is also an obstacle to gene functional analysis for reproduction traits.
Although overexpression of FLOWERING LOCUS T (FT) promotes precocious flowering of various fruit tree species, FT overexpression was unable to promote persimmon flowering in previous studies.
In this study, we tested if suppression of CENTRORADIALIS-like (CEN-like), a flowering suppressor, could induce precocious flowering in persimmon.
We conducted phylogenetic analysis to identify CEN orthologs and found two DkCEN1 and DkCEN2 as candidate genes for the flowering suppressor in persimmon.
CRISPR-Cas9 and RNA interference (RNAi) were utilized to knock out and knock down CEN-likes, respectively.
Two kinds of gRNA targeting DkCEN1 and DkCEN2 were designed for CRISPR-Cas9-mediated gene editing.
Green fluorescent protein (GFP) gene was also incorporated into CRISPR-Cas9 vector to monitor transgene expression.
For RNAi, we designed three different amiRNAs, which targets DkCEN1, DkCEN2, and both DkCEN1 and DkCEN2, respectively and placed them together on the T-DNA region of a binary plasmid.
After confirming the efficacy of binary vectors in tobacco (Nicotiana tabacum) transformation, persimmon is now being transformed.
We also tested the use of a synthetic cytokinin 4PU30 in place of usually used natural cytokinin zeatin to improve regeneration efficiency of persimmon.
The results obtained in this study will lead to better understanding of genome-editing and flowering in persimmon.

Publication

IV Asian Horticultural Congress – AHC2023

Authors

C. Iwami, M. Omori, T.-F. Hsiang, S.S. Sugano, T. Akagi, R. Tao

Keywords

YMA, Agrobacterium, artificial miRNA, gene editing, RNAi, transformation

Full text

https://doi.org/10.17660/ActaHortic.2024.1404.87

Groups involved

Online Articles (205)

1. Metabolic adaptation and fruit quality formation of citrus auto- and allo-tetraploids

2. Genome information and its industrial applications for plants

H. Hirakawa | M. Hasegawa | N. Yokotani | S. Isobe

3. Seed dormancy of Andrographis paniculate (Burm. f.) Wall. ex Nees and its relationship with seed maturity stages and after ripening period

B.T. Gundala | E. Widajati | S. Ilyas

4. Profile of gene expression at the berry enlargement phase of the large berry mutant of ‘Delaware’ grape

H. Ishikawa | Y. Togano | T. Shibuya

5. Non-destructive monitoring of maturity of peach fruit and grape berry on trees with a portable vibrating-device

N. Sakurai | T. Hino | M. Kawamura

6. Changes in ‘SunGold’ skin topography induced by controlled scuffing

T. Sneddon | S. Rivera | M. Li | J. Heyes

7. Productivity and labor saving effect of Japanese pear in ‘Joint V-shaped trellis’

T. Seki | K. Katayama | T. Soneda | K. Shibata

8. Improved methods to estimate the optimized inflorescence size for ‘Rose Red’ litchi fruit set

Jen-Yu Chang | L. Tang | Yi-Ting Zhang | Jer-Way Chang

9. Phenological observations of fruit development and ripening progression of rabbiteye blueberry

Y. Bao | H. Yamane | R. Tao

10. A spectral imaging predicting model for chilling stress of papaya (Carica papaya L.) plant

Y.C. Yeh | P.A. Chen | Y.H. Lin

11. Alleviation of high temperature stress in longan production using a hydrocooling approach

12. Differences in artificial light spectrum using light emitting diode on secondary metabolite profiles in Kasturi mango seedlings from Tembilahan, Riau, Indonesia

P. Ariansah | D.P. Hapsari | E. Inoue | R. Poerwanto | D.D. Matra

13. Induction of anti-inflammatory and antioxidant activities of ‘Mahachanok’ mango influenced by high hydrostatic pressure

S. Setha | M.Y. Thwe | P. Suthiluk | M. Naradisorn

14. The respiration and ethylene production rate on different fruit stages and cultivars of Indian jujube (Ziziphus mauritiana Lam.)

H.L. Chen | J.H. Kuo

15. Evaluation of transpiration rate of ‘Nom Sod’ jujube during the fruit setting period

S. Isarangkool Na Ayutthaya | S. Meetha | S. Nampila

16. ERAB Kaki, a database for collecting and managing persimmon breeding data and its application to the assessment of breeding progress

17. Quantitative trait locus analysis of the F₁ breeding population of 180-1-08 × ‘Del Monte Gold’ pineappleses

18. Indonesian pummelo (Citrus maxima (Burm.) Merr.): its cultivars and characteristics

19. Volatile metabolomic and transcriptome reveals terpenes release rhythm and molecular mechanisms of Rosa yangii

L.J. Zhou | S.H. Wu | M.Q. Yi | C. Yu | Q.X. Zhang

20. Functional analysis of betalain biosynthesis genes in Portulaca umbraticola

C. Sumi | M. Ishimori | M. Shibata | Y. Higuchi

21. Flavonoids bioactive content of Indigofera tinctoria as a potential medicinal plant with inorganic fertilizer applications

N.I.D. Arista | S.A. Aziz | A. Kurniawati

22. Evaluated crop water requirement of flowering potted Curcuma hybrids ‘Doi Tung Red’ under off-season production

C. Seelatlao | A. Dongsansuk | S. Isarangkool Na Ayutthaya | P. Hongpakdee

23. Classification of ethylene sensitivity in cut flowers of dahlia and selection of ‘Eternity Shine’ with low ethylene sensitivity

T. Fujimoto | T. Onozaki

24. The 1-methylcyclopropene (1-MCP) short-term treatment at room temperature enhances flower quality of cut Eustoma grandiflorum [Raf.] Shinn.

Y.H. Chen

25. Transcriptome analysis reveals senescence-related molecular mechanisms in Ornithogalum thyrsoides florets

26. Effect of fertilization in drill-holes on soil gas exchange, apple root, and fruit characteristics

Tuo Ji | Weiwei Zhang | Hongqiang Yang

27. Continuous N fertilization immobilized nuclear accident-derived ¹³⁷Cs in apple orchard soil during 10 years after the accident

K. Matsuoka | M. Igura | N. Moritsuka | T. Anazawa

28. Can cattail fibers effectively replace peat in soilless growing media?

P. Singh

29. The first step to organic hydroponics – preparing microbial culture solutions with easily accessed household liquid organic fertilizers in a subtropical farm

T.I. Lee | Y.H. Hsieh | Y.Y. Hsieh | H.Y. Chang

30. Structure and function of the xylem of tomato stem with adventitious roots

31. The effect of Burkholderia spp. inoculation on the growth of Lactuca sativa (‘Frillice Iceberg’) under waterlogging in hydroponic system

T. Weeplian | R. Manopack | P. Tiyayon | A. Vangnai

32. Effects of daytime LED supplemental lighting on strawberry growth and yield under subtropical climate

M. Nakayama | S. Fujita | Y. Nakazawa

33. Effects of the red light/far-red light ratio on plant shape, fruit yield, and fruit quality in Fragaria × ananassa Duch. ‘Beni hoppe’

34. Effect of blue LED growing light on enhancing phytonutrients and antioxidants of plant-factory grown kale microgreen

N. Pongprasert | P. Lekkham | S. Yodsarn | V. Srilaong

35. Spectrum radiometric approach: a non-destructive method for measuring the canopy light interception rate in crops

Y. Iwasaki | A. Tamura

36. Evaluation of heat stress of workers using wearable biosensors in protected horticulture

Y. Isoyama | M. Nakashima | M. Kaneko | K. Kiyono

37. The E-textile data gloves for peach harvesting

D. Miyata | Z. Yu | D. Takata | H. Saito | L. Jing

38. Study on remote farming guidance in urban agriculture utilizing 5G for local area network and IoT devices

39. Effects of planting densities on the growth of lettuce (Lactuca sativa) under continuous lighting by blue, red and far-red light emitting diodes

W. Miyazaki | N. Fukuda | H. Yoshida

40. An evaluation of the ventilation performance using a new structure of plastic low tunnels

Y. Yoneda | H. Kawashima

41. A study on okra (Abelmoschus esculentus) and thanksgiving cactus (Schlumbergera truncata) for the introduction as rooftop greenhouse plants

S.Y. Lee | S.H. Jung | Y.J. Kim

42. Latest developments in Taiwanese green care initiatives and the lessons Japanese counterparts can learn

H. Tsunashima

43. Effect of light environment controlled by red and blue LEDs on the growth of Lactuca genetic resources

44. A GRAS-Di system linkage map in Chinese cabbage

H. Mehraj | M.A. Akter | T. Takasaki-Yasuda | E.S. Dennis | R. Fujimoto

45. Expression analysis of flowering gene in diploid wild strawberry species

T. Kurokura | M. Shibuya

46. Agrobacterium-mediated transformation of rose meristem in vitro

47. Induction of calluses from petal of three chrysanthemum cultivars in vitro

S. Boonprasop | K. Saetiew | M. Teeralak

48. Novel aspects and future directions of the rol-technology: improving plants through a non-GMO breeding method

H. Lütken | X. Chen | J. He | B. Trevenzoli Favero

49. Effects of organic compound and plant growth regulators on seed germination and growth of Doritis pulcherrima Lindl. in vitro

S. Supreeworakij | K. Saetiew | T. Rungkitwattananukul | P. Phanchompoo | N. Montri

50. Transcription factors involved THCAS promoter in cannabinoid biosynthesis pathway of Cannabis sativa L.

51. Evaluation and analysis of different types of pear flesh by TPA during shelf life

52. Gaseous ozone as a postharvest treatment: recent lessons from South Africa

A. Mditshwa | N.L. Bambalele | N.H. Gcabashe | S.Z. Tesfay | L.S. Magwaza

53. Impact of high-pressure on enzyme activity involved in enzymatic browning characteristics of fresh cut apple

J. Phakawan | R. Tepsorn

54. Curing acceleration as an adaptive postharvest strategy for mid-autumn festival associated irregular harvest date of ‘Matou Wentan’ pomelo in Taiwan

L.-Y. Chang | W.-C. Chang

55. Effects of preharvest UV irradiation combined with postharvest storage temperature and low light irradiation during storage on the functional compounds of komatsuna (Brassica rapa var. perviridis)

S. Hikosaka | M. Harada | H. Yoshida | E. Goto

56. Development and evaluation of hinokitiol active paper sheets for the postharvest management of broccoli

K.H. Keerthana | N. Benyakart | H. Umehara | T. Vanitha | T. Shiina

57. Effect of electric field treatment on postharvest quality of green bell pepper

Y. Hamaguchi | R. Yoshida | D. Hamanaka

58. Preharvest treatment to increase postharvest quality of hot pepper

59. Effect of sugar-ester edible coating on postharvest life and development of chilling injury in tomatoes during ambient and cold storage

60. Shelf-life extension of fresh-cut mango (Mangifera indica L. ‘Nam Dok Mai Si-Thong’) by coating with calcium lactate and ascorbic acid stored in active modified atmosphere packaging

T.N. Aye | M. Naradisorn | T. Pranamornkith

61. Extracting strawberry anthocyanins, testing different variables

T. Taghavi | H. Patel | A. Rahemi

62. Production of cinnamon essential oil-loaded electrospun nanofiber for postharvest technology application usage

63. Identification of candidate genes associated with the defense response following Albugo candida inoculation in Brassica rapa L.

64. Rapid screening of potential plant-growth rhizobacteria from soils to control chili wilt disease and to promote growth and yield of chili

P. Tiyayon | R. Manopack | T. Weeplian | W.J. Liang

65. Stem rot of Indian borage caused by Phytopythium helicoides in Taiwan

H.J. Chien | C.H. Lin | C.H. Kuo

66. Seed biopriming or coating with rhizobacteria on hot pepper (Capsicum annuum L.): its effects on plant growth, yield, and incidence of Phytophthora blight

S. Ilyas | I. Madyasari | C. Budiman | D. Manohara

67. Nuti-priming for enhancing seed germination of Prosopis cineraria for afforestation of deserts

B.M.S. Alhebsi | D.V. Francis | Z.F.R. Ahmed

68. Germination characteristics of Angelica gigas and Angelica acutiloba as affected by cold stratification and hot water treatment before sowing

69. Solanum rootstock biodiversity to deal with holoparasitism and fertilizer use efficiency

70. Growth characteristics and physiological disorders of cucumber grafted seedlings exposed to low temperatures

71. Growth control of Platycodon grandiflorum seedling using difference between day and night temperatures

72. Evaluation of cabbage growth using quadratic distribution under soil moisture regimes

M.Z.K. Roni | M. Fukuda | F. Sato

73. Upgrading the cocoa value chain in Nan province based on local resources and circular economy principles

T. Na Nan | A. Feepakpro | S. Thongpun | V. Nortow | T. Kakham

74. Persimmon cultivation in Afghanistan: from import to local production

Z. Hejazi | M.I. Sadat | T. Tetsumura

75. Shifts in the American floriculture industry: comparing international and domestic growers

E.B. Jenkins | M.J. Knuth | C.R. Hall | M.A. Palma

76. Japan’s flower and plant industry during the COVID-19 pandemic

Y. Niisato

77. Identification of a major QTL controlling onion bulb shape

D. Sekine | H. Tsukazaki | S. Oku

78. Estimation of candidate genes for chemical components involved in dark green coloration of bunching onion by QTL analysis

79. Development of a QTL marker for quercetin glycoside content in onion

80. A unique chiasmata formation observed in a certain type of Allium monosomic addition lines and estimation of its causal genes

81. Polyploidy induction and identification of Lilium ‘Siberia’

Q. Zhang | L.X. Wu | X.O. Ma | Y.C. Tang | M. Sun

82. Genetic diversity of marigold (Tagetes patula L.) mutant treated by induced mutation with gamma rays irradiation

83. MARCO: a computer software for parentage inference using molecular markers

84. Inhibition of in vitro pollen tube growth in ‘Hyuganatsu’ (Citrus tamurana hort. ex Tanaka) by recombinant S₁₅-RNase protein

C. Honsho | T. Inaba | Y. Sugimoto | S. Ishimura | T. Tetsumura

85. Development of a database program for managing genotype information and simulating segregation patterns of horticulturally important genes in apple

S. Moriya | T. Shimizu | A. Hori | Y. Sawamura

86. Quantification of the diverse temperature requirements for blooming in Prunus mume Japanese collection

Y. Kitamura | T. Kashiwamoto | T.-F. Hsiang | K. Numaguchi | H. Yamane

87. Identification and expression analyses of the CENTRORADIALIS (CEN) gene family involved in flowering regulation in persimmon (Diospyros kaki)

88. ‘Suhyang’: new strawberry cultivar from SRI, Republic of Korea

89. Varietal differences in the content of strawberry allergen Fra a 1.01 among Japanese cultivars

90. Development of a new winter squash cultivar ‘Houchouko 1’ with short internodes and stable fruit position

91. Remapping the precise genomic positions of two loci (Shql3 and Shql7) associated with the reduction of pungency in chili pepper (Capsicum annuum)

F. Kondo | K. Umeda | K. Nemoto | K. Matsushima

92. Bulked sequence analysis identifies two loci controlling anthocyanin accumulation in a red-purple cultivar of Japanese leaf vegetable komatsuna (Brassica rapa L.)

93. QTL analysis of earliness traits for bulbing in onion

H. Tsukazaki | S. Oku | S. Hiura | D. Sekine

94. Embryo age and culture medium determine the efficacy of embryo rescue in hybridizations between Crossostephium chinense (L.) Makino and Chrysanthemum spp.

95. Stepwise improvement of flower color by synchrotron-light re-irradiation in pink-flowered standard chrysanthemum

K. Sakamoto | T. Takamura

96. Effects of five types of ion beam irradiation on the dry seeds of Gerbera hybrida

T. Hosoguchi | M. Yahata | T. Shimokawa | A. Tominaga

97. Unique ornamental strawberry cultivar ‘MS1615-01’ with fully double flowers

Y. Noguchi | S. Kataoka | S. Ezawa | Y. Maeda | F. Tanimura

98. Effect of storage temperature and 1-MCP treatment on fruit ripening in Japanese apricot fruit (Prunus mume)

99. Aloe vera and chitosan coating, an eco-friendly and sustainable approach to reduce postharvest loss of figs (Ficus carica)

N. Kaur | C. Somasundram | Z. Razali | Z.F.R. Ahmed

100. Wrapping materials and their effect on postharvest performance of ‘Femminello Siracusano’ lemon

Z. Hejazi | A. Zerak | T. Zahid

101. Irradiation as a quarantine treatment on the quality and sensory evaluation of ‘Thong Dee’ Thai pomelo (Citrus maxima (Burm.) Merr.) during storage

P. Wichitkunanan | S. Jetawattana | H. Shamsub | P. Chaiprasart

102. The quality of paprika (Capsicum annuum L.) by temperature changes on the simulated export condition

M.R. Kim | J.H. Lim | J.S. Lee | S.B. Yang | C.S. Kang

103. Influence of gibberellic acid on leaf abscission and quality of sweet basil

P. Yingsanga | A. Srikram | P. Jitareerat | K. Sripong | T. Srinon

104. Enzymatic browning inhibition of pomegranate extract incorporated in liposome encapsulated on fresh cut yacon tuber

J. Phakawan | R. Tepsorn

105. Effect of water supply material volume and leaf area during transportation on the quality of cut dahlia flowers

N. Tsujimoto | K. Inda | Y. Harada

106. Development of new dahlia cultivars ‘Eternity Peach’ and ‘Eternity Shine’ with long vase lives

T. Onozaki | T. Fujimoto | M. Azuma

107. Calcium carbonate delays the senescence of cut waterlily flowers

I. Lichanporn | N. Nanthachai | P. Tanganurat | P. Akkarakultron

108. Effects of pre- and postharvest LEDs treatments on physio-biochemical properties of roselle (Hibiscus sabdariffa L.) microgreens: a preliminary study

S. Phornvillay | N. Pongprasert | V. Srilaong

109. Effects of methyl jasmonate on the flower opening of cut spray rose flowers

K. Hamada | A. Inoue | K. Kurachi | Y. Horaguchi | T. Horibe

110. Anthocyanin accumulation in the flesh of ‘Ruby Sweet’ and ‘Rose Pearl’ apples during cold storage

C. Honda | R. Fujiwara | K. Okada | M. Tatsuki | H. Iwanami

111. Transcriptome analysis for ‘Ruby-Roman’ grapes grafted on diploid and tetraploid rootstocks

112. Effects of location planting on the quality and antioxidants of ‘Namwa’ bananas produced in northeastern Thailand

S. Nampila | S. Meetha | S. Kanjanawattanawong | S. Isarangkool Na Ayutthaya

113. Histochemical studies of aroma components in fruits of wild tomato species native to the Peruvian Andes and the Galapagos Islands

T. Kobayashi | A. Kondo | R. Kawazura | H. Hosono | T. Tabuchi

114. Effect of environmental factors, growth traits, and chlorophyll a fluorescence OJIP transients on lutein content of spinach (Spinacia oleracea) for frozen processing

T. Nakamura | K. Zushi

115. Inheritance of peonidin 3-O-neohesperidoside synthesis in the petals of cyclamen

T. Takamura | Y. Terada

116. Expression analysis of anthocyanin biosynthesis-related genes in bicolor Echinacea ‘Pink Eye’

M. Kojima | N. Noda | T. Sakagami | Y. Akita

117. Functional analysis of flavonoid 3’,5’-hydroxylase gene using transgenic Japanese morning glory (Ipomoea nil)

118. Role of flavonols in the pale-yellow flower color expression of Ranunculus cultivars

Q. Qin | F. Tatsuzawa | T. Nakane | T. Iwashina | T. Mizuno

119. Analysis of floral scent components of roses with unpleasant odor

N. Oyama-Okubo | Y. Mikanagi

120. Study on the floral fragrance components and osmophore groups of modern rose ‘White Lychee’

Y.Y. Chen | L.J. Zhou | C. Yu

121. Comparison of the scent components emitted from lotus (Nelumbo nucifera) and American lotus (N. lutea) flowers

Y. Ishikawa | F. Tanaka | N. Oyama-Okubo | C. Honda

122. Crude polysaccharides from orchid pseudobulbs modified the in vitro development of Dendrobium cretaceum and its antioxidant activity

K. Obsuwan | K. Rayanil | N. Sanmanee | A. Charoenpanich | S. Maksup

123. An analysis of the levels of bioactive components and antioxidant activity in several Dendrobium ‘Khao Sanan’ sections taken at various developmental stages

S. Maksup | K. Obsuwan

124. Resin canal discoloration of mango fruit: impact of sanitizer, harvest time and presence of fruit peduncle at harvest

C.A. Asis | A. Niscioli

125. Growth promotion and disease tolerance in strawberry plants with plasma-activated Ringer’s lactate solution

126. Genomic analysis of the region including transcription factor PavMYB10.1 and development of DNA markers for yellow pericarp of sweet cherry

M. Sato | F. Tada | K. Ikeda

127. Occurrence pattern of diseases and pests in strawberry greenhouse during harvest period

128. The recent occurrence pattern of diseases and pests in strawberry greenhouse of Chungnam province, South Korea, during nursery period

129. Progress in the development of anthracnose-resistant F₁ hybrid strawberries using self-pollinated parental lines generated by recurrent selection

130. Evaluation of Cucumis zambianus as a genetic resource for Fusarium wilt resistance in melon

M. Yamaga | Y. Matsumoto

131. Development of melon F₁ hybrids with resistance to cucurbit chlorotic yellows virus

132. Evaluation of processing tomato resistance to tomato yellow leaf curl virus (TYLCV) and detecting Ty-2 and Ty-3 resistance genes with SCAR-markers

133. Suppressing effects of paper pot transplantation and sulfur material application on southern blight on Jerusalem artichoke

Y. Matsumoto | M. Yamaga | T. Watanabe

134. Evaluation of chili (Capsicum annuum L.) progressive lines for resistance against Colletotrichum capsici and Colletotrichum gloeosporioides

135. Individual differences in disease progression of rose crown gall disease in a cross population between tetraploid Rosa multiflora and R. ‘PEKcougel’

M. Ochiai | D. Maruyama | J. Haruyama | K. Yamada

136. Growth promotion and increase in functional constituents through arbuscular mycorrhizal fungi and herbal extracts in Platycodon grandiflorus

X. Qin | Y. Matsubara | T. Ozeki | D.K. Watanabe

137. Evaluation of Bacillus spp. for biological control of Fusarium stem rot in Anoectochilus formosanus

C. Chen | C.Y. Lu | R.M. Hsieh | G.M. Yang

138. Trehalose and forchlorfenuron reduced the time required for picking berries but not bunch quality in the hybrid grape Vitis labrusca × V. vinifera ‘Shine Muscat’

139. Isoprothiolane regulates endogenous abscisic acid and indole-3-acetic acid during grape berry maturation

140. Multi-year comparison of normal and split-pit peach quality

141. Assessing the effects of high temperature on physiological disorders in peach fruit (Prunus persica L.)

142. Early flowering bud-sport mutant derived from Japanese apricot ‘Nanko’

M. Sugimori | T.-F. Hsiang | H. Yamane | Y. Kitamura | R. Tao

143. Gibberellic acid modulates the expression of flowering-related genes and the flower bud rate in apple (Malus domestica)

144. Typing and distribution of the SFBB alleles encoded by four S-haplotypes of European pear (Pyrus communis L.)

K. Tadokoro | M. Kobayashi | R. Fujimoto | T. Takasaki-Yasuda

145. Rooting of softwood cuttings of ‘Porotan’ Japanese chestnut

146. Effects of soil water content and light intensity on physiological responses and mineral absorption in passion fruit

Z. Arisawa | T. Kondo

147. The natural flowering inhibition of aminoethoxyvinylglycine application with different schedules for pineapple autumn fruit production in Taiwan

Shih-Huang Huang

148. Effect of parthenocarpic fruit development on the occurrence of severe microcracking in cherry tomato

R. Hosoi | K. Ikeda | T. Nishizawa | T. Nabeshima

149. Using radiation thermography to evaluate high temperature stress in cauliflower

Chiao-Ling Hsiao | Chen-Yu Lin

150. Effects of air and root zone temperature on the distribution of ¹³C photosynthates in asparagus plants

S. Watanabe | S. Moriyuki | K. Matsuo | K. Hidaka | Y. Nakajima

151. Differential response and sensitivity to exogenous ethylene of cut flowers of two Vanda hybrids – ‘Sansai Blue’ vs. ‘Kanun Blue’

M. Buanong | S. Khunmuang | C. Wongs-Aree | S. Meir | S. Philosoph-Hadas

152. Factors affecting organogenesis of terrestrial Cymbidium species in vitro

K. Shimasaki | S.J. Nahar | M.M. Kamal | A.F. Ona

153. Effects of temperatures on seed germination and initial growth of seedlings in Wisteria floribunda

Y. Mizunoe | S. Sadasue | Y. Ozaki

154. Effect of humic substances and mycorrhizal symbiosis on growth and heat stress tolerance in everbearing strawberry

S. Sawamura | Y. Matsubara | N. Terai | Y. Takeshita | S. Nakano

155. Effect of bulbs size and gibberellic acid (GA₃) on growth and flowering of Eucomis

K. Panjama | S. Ruamrungsri | C. Inkham

156. Characteristics of Iris ensata var. spontanea growing along the Tanesashi Coast, Japan: focusing on salt tolerance

M. Nakagomi | T. Kobayashi | Y. Sadanobu | T. Tabuchi | K. Matsumoto

157. Vernalization characteristics in Lavandula × intermedia

158. Large amounts of calcium oxalate crystals accumulate in epidermis of edible cactus (Nopalea cochenillifera)

T. Horibe | M. Mamiya | Y. Miyashita | R. Yamaguchi

159. Promotion of rooting and growth with the changes in functional metabolites in mycorrhizal tea plants

160. Effect of BA and IBA on micropropagation of Cannabis sativa L. ‘Hang Kra Rog’

P. Singhavorachai | R. Deewatthanawong | N. Montri

161. A preliminary study on image-based grading of apples harvested in the southern region of Nagano Prefecture, Japan

Y. Osako | M. Hattori | K. Okada

162. Effect of ‘joint cutting’ and positional difference on new shoot growth and uniformity of fruit quality in tree joint training system of Japanese pear ‘Housui’

K. Iimura | Y. Sekozawa | S. Sugaya

163. Productivity and productive age of tree joint training system of Japanese pear as high-density planting cultivation without dwarfing rootstock

K. Shibata | K. Hirose | T. Soneda | K. Katayama | T. Seki

164. Toward an establishment of a deep learning-based flower bud phenotyping system in Prunus mume

T.-F. Hsiang | Y.-R. Lin | H. Yamane | S. Nishiyama | R. Tao

165. The timing of trehalose injection affects the number of flowers and the expression of VvCKX5 in the hybrid grape Vitis labrusca × V. vinifera ‘Shine Muscat’

166. Effective pollination period of ‘Fuji Murasaki’ Akebia trifoliata

D. Matsumoto | K. Ito | S. Taira

167. Effects of soil pH in Andosols on growth and mineral absorption in passion fruit

T. Kondo | K. Minowa

168. Evaluation of tools for pollination of strawberry production in a plant factory with artificial lighting (PFAL)

Y. Nishimoto | N. Lu | M. Takagaki

169. Effects of environmental conditions for inducing flower bud initiation on plant growth and flowering of June-bearing strawberry grown under LED lighting

170. Reproduction of radiative cooling in a greenhouse using a hot box with a thermal plant model

Y. Ohashi | R. Tsuchiya | M. Ishii | M. Hayashi

171. Estimation of strawberry fruit weight based on fruit diameter

172. Effect of carbon dioxide application on tomato growth dynamics and yield within a low solar radiation environment in Akita (cold region), Japan

173. Prediction of yield based on fruit set and dry matter production in greenhouse tomato

M. Itoh | Y. Muramatsu | D.H. Ahn | T. Higashide

174. Effects of environmental control using groundwater and full open-roof vinyl house on yield in long-term cultivation of three cultivars of tomatoes in Akita, one of the coldest regions in Japan

175. Effects of sorghum green manure crop on yield and root zone of ‘Mizunasu’ eggplant

H. Akrich | R. Arakawa | N. Tokuno | S. Segami

176. Modeling fruit set in greenhouse sweet pepper

M. Homma | D.H. Ahn | T. Higashide

177. Effect of different leaf area management on growth and yield of cucumber (Cucumis sativus L.) cultivated in hydroponic system

K. Maeda | K. Nomura | D.H. Ahn

178. Effect of substrates elaborated with waste of tires and cables in lettuce growth

179. LCA analysis of leaf lettuce production in plant factories in various decarbonization scenarios

S. Kawabata | Y.C. Lan

180. Bioaccumulation of heavy metals from substrates elaborated with waste of tires and cables in lettuce

P.A. Casquero | A. Porteous | C. Domínguez | A. Rodríguez-González | S. Mayo-Prieto

181. Effects of rain-protected culture in a plastic greenhouse using the whole harvest cultivation method for one-year-old asparagus plants

S. Yamashita | K. Seimiya | K. Yamafuji | T. Taguchi | S. Motoki

182. Estimation of the light extinction coefficients of lateral asparagus ferns using a numerical method

T. Kuroyanagi | H. Yoshikoshi | M. Hikawa-Endo

183. Glycine betaine material affects the growth and yield of asparagus in the long-term harvest production system in a semi-forcing culture

F. Satake | K. Okada | Y. Yoshida | W. Sakata | S. Motoki

184. Mounding increases the rate of thick spears of asparagus

K. Suzuki | Y. Ayabe | A. Kato | S. Motoki

185. Investigation of nutrient solution management in hydroponics using organic fertilizer

T. Endoh | M. Takagaki | P. Suwitchyanon | C. Chaturong

186. Effect of different cultivation methods and environments on the growth and yield of Shimonita-Negi

M. Okabe | K. Obokata | T. Hirose | Y. Hashida

187. Predicting corn salad (Valerianella spp.) weight in a plant factory with artificial lighting (PFAL) using K-Nearest Neighbors algorithm (KNN)

S. Dhammayorddee | C. Chanseetis | A. Kato | M. Takagaki | N. Lookin

188. The effect of water and fertilizer types on growth and yield of supan-supan (Neptunia oleracea) cultivated on flooded Ultisol media

H. Susanti | J. Purnomo | O.A. Kumadawara

189. Effect of low sunlight and high temperature during flower bud differentiation on the cut flower quality of carnation ‘Cherry Tessino’

S. Kamachi | R. Sakai | S. Nagashima | S. Mori

190. Deficit irrigation and fertilizer rates affecting growth, flower quality and physiological changes in tuberous begonia grown in plant factory

C. Inkham | K. Panjama | S. Ruamrungsri

191. Growth and flowering characteristics of Calendula under different light spectra in a controlled environment

M. Munyanont | N. Lu | T. Joilek | D.F. Rachma | M. Takagaki

192. Selection of optimal marigold (Tagetes patula) cultivar as an edible flower in a plant factory

N.Y. Kwak | B.H. Sung | K.P.S. Kumaratenna | Y.Y. Cho

193. Promoting flower yields, photosynthetic pigments and some secondary metabolite in Marguerite daisy (Chrysanthemum frutescens L.) cut flower under the smart farming system by pinching technique

Y. Luekhampa | C. Seelatlao | W. Chareontham | J. Sanitchon | P. Hongpakdee

194. Nutrient deficiencies affect growth and development of vinca

S. Ruamrungsri | C. Inkham | K. Panjama

195. Relationship between Asian ginseng decline and soil chemical property

Md.A.A. Mahmud | Y. Matsubara | T. Ikezawa

196. Influence of different flower vases on the impression of cut flowers

Y. Mitarai

197. Effects of different LED light spectrum on growth and cannabinoid contents of Cannabis sativa L. hybrid ‘KKU’ cultivars under an indoor pot cultivation

P. Nounbunma | P. Hongpakdee | J. Paluka | C. Lapjit

198. Effects of cultivation environmental factors on the growth of the edible cactus (Nopalea cochenillifera) grown under hydroponic conditions

S. Tsuchimoto | K. Okada | K. Kondo | T. Horibe

199. Calcium and magnesium biofortification of the edible cactus Nopalea cochenillifera grown under hydroponic conditions

E. Nishimura | K. Hayashi | T. Horibe

200. Effect of selling area, period, and type on the price of okra (Abelmoschus esculentus L.) in Japan

L. Sato | N. Miyamoto | S. Motoki

201. Comparative analysis of asparagus marketing practices in Europe, North America, and Asia during summer and autumn

S. Yamashita | S. Motoki

202. Edible alleys in the discourse of urban horticulture: a case study on densely built urban renewal districts in Chiayi, Taiwan

T.I. Lee

203. Application of calcium chloride on yield and quality of Capsicum annuum L.

N. Montri | S. Sengseng | R. Deewatthanawong | K. Bunya-atichart

204. Development of a growth state estimation system using a moving camera in strawberry cultivation

S. Matsuo | H. Umeda

205. The impact of adding nanoparticles to the media to speed up the in vitro multiplication stage of banana explants

P. Nugrahani | I.R. Moeljani